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Assessment of Cellular GSH and GSSG Content To investigate the GSH and GSSG content in GSTP1 - and mock-transfected HepG2 cells, concentrations of total GSH and GSSG were measured using the GSH/GSSG-Glo TM Glutathione Assay kit (Promega, Madison, WI, United States)

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It is also essential to fully assess the unforeseen side effects of long-term NAD + boosting