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The PVDF membranes were blocked with 5% nonfat milk for 1.5 h and then incubated overnight at 4 C with the following primary antibodies: anti-TNF- (Abcam, ab300093), anti-NLRP3 (Abcam, ab254360), anti-BDNF (Abcam, ab108319), anti-Synaptophysin (SYP) (Abcam, ab156302) and anti-PSD95 (Abcam, ab238135)

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In another study, rats with diabetic kidney disease (DKD) were treated with a CD38 inhibitor, resulting in increased renal NAD + levels, which in turn decreased mitochondrial oxidative stress 144

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