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These mechanisms are implicated in immune regulation, development, and age-related pathologies, thus highlighting the broad physiological importance of DNA methylation in the control of autophagy

In brief, cells seeded in 6- well plates (3000 cells/well) were dosed 24h later and continually treated with rapamycin for 7 days (refresh drugs every three days), the resulting colonies were stained with crystal violet (0.5% dissolved in 25% methanol)

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