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6 Other dried aliquots of the fraction (300 L) were treated with 50 L of 20 mM NEM in PBS for 10 min

The other family members and carriers took NAC as a dietary supplement (i.e., purchasing NAC online through Amazon) based on the results from the cell-based assays, while waiting to formally enroll into a clinical trial that was designed to assess the efficacy of NAC in patients with HCCAA and launched in 2019